Stability Of Syphilis Freezer Samples

Stability Of Syphilis Freezer Samples      Syphilis is a sexually transmitted disease that is caused by the bacteria Treponema pallidum. It can be diagnosed by using many different types of tests such as the RPR test (Rapid Plasma Reagin test), VDRL test (Venereal Disease Research Laboratory test), TPHA test (Treponema pallidum haemagglutination assay test) or the FTA-ABS test (Fluorescent Treponemal Antibody Absorption test).

     There is yet another quick and accurate test which is known as the Syphilis Rapid Card Test. This test can be performed using human serum.

     For this, the individual’s blood sample is collected and stored in a sterile container without adding any anticoagulants. The blood is allowed to clot and the plasma and serum are separated. The serum is then used for the test. The serum should not be left unused at room temperature for prolonged period of time. In case the serum sample cannot be tested immediately on the same day that the blood sample is collected, then it must be stored in a freezer.

      The serum sample can be maintained at 2° to 8° C for a maximum of 3 days. However, if the serum sample needs to be retained for longer duration than this, then for such long-term storage, the serum sample should be stored in a freezer and the temperature must be maintained below –20° C

      However, the serum cannot be tested in this frozen state. Therefore, the frozen serum sample must be completely thawed before use. The serum sample must be carefully brought back to normal room temperature and mixed well before the testing. Repeated freezing and thawing of the serum sample should be avoided.

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Stability Of Syphilis Freezer Samples